TABLE 1.

Calculated parameters based on two-step analysis of the data shown in Figs. 2 and 3 describing the irreversible thermal transitions of holo- and apo- forms of human CP

CP form	Ea1 (kJ/mol)	T*1 (K)	ΔH1 (kJ/mol)	Ea2 (kJ/mol)	T*2 (K)	ΔH2 (kJ/mol)	R	Tobs† (°C)
Apo								1st/2nd
DSC 0.5 K/min	222	332	407	275	334	1571	0.9980	50.9/55.5
DSC 1.0 K/min	273	328	365	326	333	1593	0.9975	52.7/57.0
DSC 1.5 K/min	267	330	394	323	333	1401	0.9994	53.9/58.0
Average	254	330	389	308	333	1521
FTrp 0.5 K/min	238	334	–	240	334	–	0.9999	54.1
FTrp 1.0 K/min	274	334	–	331	335	–	0.9997	55.6
FTrp 1.5 K/min	239	334	–	322	331	–	0.9999	56.6
CD 0.5 K/min	181	341	–	176	335	–	0.9991	55.3
CD 1.0 K/min	167	341	–	298	332	–	0.9985	57.9
CD 1.5 K/min	187	340	–	265	332	–	0.9979	59.9
Holo
DSC 0.5 K/min	268	334	420	219	353	1917	0.9934	55.0/71.6
DSC 1.0 K/min	219	335	443	230	351	1801	0.9989	56.5/72.4
DSC 1.5 K/min	275	333	381	232	351	1930	0.9990	57.4/74.0
Average	254	334	415	227	351	1883
FTrp 0.5 K/min	275	334	–	175	358	–	0.9996	54.1/70.0
FTrp 1.0 K/min	278	333	–	213	354	–	0.9998	56.4/72.6
FTrp 1.5 K/min	232	336	–	221	355	–	0.9998	58.5/75.9
CD 0.5 K/min	174	347	–	210	357	–	0.9995	56.7/71.1
CD 1.0 K/min	152	348	–	235	354	–	0.9986	59.0/73.1
CD 1.5 K/min	118	354	–	337	352	–	0.9987	61.6/75.0

T* values are the temperatures at which the rate constant for the specific reaction is 1/min. Index 1 corresponds to the first N-to-I transition, and index 2 corresponds to the subsequent I-to-D process. The DSC data are most reliable and were used to derive average values for each parameter. R is the correlation coefficient (see Eq. 4).

^†Observed thermal midpoints; for some apo-CP data, only the midpoint of the major transition is reported.