Skip to main content
. 2007 Oct 12;94(4):1483–1496. doi: 10.1529/biophysj.107.111674

FIGURE 2.

FIGURE 2

Corecruitment of Bassoon, CtBP1, and CAST to the same intracellular complexes in COS-7 cells (a–d). Cells were transfected with Cerulean-CAST (a) and Citrine-Bsn1692-3263 (b) and immunostained with antibodies against CtBP1 (c). The overlay of the trimeric complex is given in (d), where Cerulean-CAST, Citrine Bsn1692-3263, and CtBP1 are shown in green, red, and blue, respectively. (e–g) In the absence of Bassoon, the presence of CAST did not affect the nuclear localization of endogenous CtBP1. Cerulean-CAST (g) was observed to form cytosolic complexes, but no extranuclear recruitment of CtBP1 to these complexes was observed as visualized by Alexa 594 immunostainings (e).