TABLE 1.

Rate constants (25°C) and activation energies

	frq+	Frq1	Frq7	frqS513I
k3, h−1	0.05	0.15	0.05	0.05
k5, h−1 (31)*	0.27	0.4	0.15	0.1
K6, h−1	0.07	0.1	0.01	0.006

Other rate constants (frq mutant independent; experimental rate constants are indicated by an asterisk followed by a reference): k₁ = 1.8 a.u. h⁻¹; k₂ = 1.8 a.u. h⁻¹; *k₄ = 0.23 h⁻¹ (29); k₇ = 0.16 a.u. h⁻¹; k₈ = 0.8 a.u. h⁻¹; k₉ = 40.0 a.u. h⁻¹; *k₁₀ = 0.1 h⁻¹ (20); k₁₁ = 0.05 h⁻¹; k₁₂ = 0.02 h⁻¹; k₁₃ = 50.0 a.u. h⁻¹; k₁₄ = 1.0 h⁻¹; k₁₅ = 5.0 h⁻¹; K = 1 .25 a.u (describing frq⁺); K = 8.5 a.u (describing ER24); K₂ = 1.0 a.u.; a.u. = arbitrary units.

Activation energies (kJ/mol) for the temperature description of frq⁺ and ER24: E₁ = 62.6; E₂ = 20.9; E₃ = 25.4; E₄ = 15.2; E₅, curved Arrhenius equation, see Figs. S4 (Supplementary Material); E₆ = 31.9; E₇ = 104; E₈ = 22.0; E₉ = 66.2; E₁₀ = 30.0; E₁₁ = 50.6; E₁₂ = 25.4; E₁₃ = 58.6; E₁₄ = 50.2; E₁₅ = 50.4; E_K, curved Arrhenius equation, see Fig. S4 (Supplementary Material); = 68.8.