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Simultaneous detection of a mixture of two structure-related peptides in a (M113F)7 pore. (Left) Current traces. (Right) Event histograms. The experiments were carried out at +40 mV (cis at ground) under symmetrical buffer conditions with 1 M (or 4 M) NaCl, and 10 mM Tris · HCl (pH 7.5). αHL protein was added in the cis compartment, whereas 1 μM Y-P-F-F or 1 μM Y-F-F was added in the trans compartment of the chamber.
