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. 1999 Jun 22;96(13):7569–7574. doi: 10.1073/pnas.96.13.7569

Figure 3.

Figure 3

Synergy between ERP and EPO. (A) Cells were stimulated with hormone in the presence and absence of peptide at concentrations indicated. Doses of hormone and peptide were chosen so as to yield weak signals on their own. Incubation conditions, conventions, and symbols were as in Fig. 2. After cell lysis, immunoprecipitation (IP) was done with αSTAT5 antibody, Western blot analysis (WB) with αPY (Upper) or αSTAT5 (Lower). Densitometry data as multiples of unstimulated readings are presented below upper panel. (B) Observed synergistic effects of EPO and ERP. STAT5 phosphorylation at 0.01 units/ml EPO and 10 nM ERP is presented. A = hormone alone, B = peptide alone, C = observed effect of peptide and hormone together. Data are mean ± SEM (n = 3). Units on y-axis are multiples of baseline data for untreated cells.