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. 1999 Jun 22;96(13):7569–7574. doi: 10.1073/pnas.96.13.7569

Figure 5.

Figure 5

Effect of ERP on STAT5 phosphorylation at high concentrations of EPO (bell-shaped curve). (A) TF-1 cells were stimulated for 30 min at 37°C by incubation with various concentrations of EPO or ERP or both, as indicated. Conventions and symbols are as in previous figures. After cell lysis, immunoprecipitation (IP) was done with αSTAT5 antibody followed by αPY and αSTAT5 Western blot analysis (WB). Densitometry data are shown below upper panel. (B) ERP effects on STAT5 phosphorylation at high concentration (200 units/ml) of EPO. (Left) EPO bell-shaped dose-response curve with inhibition at 200 units/ml. (Right) Comparison between EPO alone at 200 units/ml (gray bars) and in the presence of different ERP concentrations (black bars). Data are mean ± SEM of three independent experiments.