Table 1.
ERP inhibits internalization of EPO-R
| Peptide | Time, min | Surface receptors, (% of total)
|
|
|---|---|---|---|
| Mean | (SEM) | ||
| None | 5 | 65.3 | (3.7) |
| 10 | 53.3 | (6.2) | |
| 20 | 55.3 | (2.1) | |
| 40 | 53.8 | (0.6) | |
| ERP, 15 μM | 5 | 78.3 | (1.4) |
| 10 | 78.5 | (3.0) | |
| 20 | 73.0 | (2.0) | |
| 40 | 81.8 | (6.1) | |
Internalization of EPO was measured in TF-1 cells as described (1). Surface receptors were determined by the difference between total specific binding of labeled EPO and residual binding after treatment at acid pH. Data are mean ± SEM of triplicates from three experiments.