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. 2004 Mar 15;199(6):805–814. doi: 10.1084/jem.20031454

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — SOST mRNA is expressed during the mineralization phase of osteoblastic cultures. Mouse MSCs (A), KS483 cells (B), and human MSCs (C) were cultured under osteogenic conditions. SOST mRNA expression during osteoblastic differentiation is shown in relation to the late osteoblast differentiation marker osteocalcin. Osteocalcin was amplified over 30, 30, and 35 cycles for mouse MSCs, KS483 cells, and human MSCs, respectively. The amplicons had the predicted size of 199 bp (mouse primer set 1), 430 bp (mouse primer set 2), and 242 bp (human primer set). SOST was amplified over 35, 35, and 40 cycles, respectively, and the amplicons had the predicted size of 137 bp (mouse primer set) and 185 bp (human primer set). KS483 cells were cultured in the absence or presence of 10⁻⁸ M PTHrP with continuous treatment from day 4 onwards. MW, 100-bp molecular weight marker. PC, positive control.