Figure 2.

CB CD34⁺ cells transduced with STAT5A(1*6) have a proliferative advantage in stroma coculture. CB CD34⁺ cells were transduced with MiGR1 or STAT5A(1*6) as described in Fig. 1 and grown in cocultures on MS5 (A and B), OP9 (C), AGM-S2 (D), or in liquid culture (E and F) in IMDM in the presence of 10% FCS, 20 ng/ml IL-3, 20 ng/ml IL-6, KL, FL, and TPO (100 ng/ml each). Cultures were weekly demidepopulated and cells were counted and analyzed for GFP expression by FACS. Data are of a representative experiment in triplicate out of at least three independent experiments. A and E represent the expansion in absolute cell counts of GFP⁺-sorted cells. B–D and F represent experiments started with unsorted cells and indicate the percentage of GFP⁺ cells. Week 0 represents transduced cells before plating on stroma or liquid cultures.