Figure 6.

RT-PCR analysis of CB CD34⁺ cells transduced with STAT5A(1*6) versus MiGR1. (A) CB CD34⁺ cells were prestimulated for 48 h in QBSF supplemented with KL, FL, and TPO (100 ng/ml of each) followed by three transduction rounds in the next 48 h with STAT5A(1*6) or MiGR1 retroviruses. Total RNA was isolated and used in RT-PCR reactions as described in Materials and Methods, and used for RT-PCR reactions. (B) RT-PCR analysis as in A, but on total RNA isolated from the week 2 nonadherent cells from MS5 cocultures.