Table I.
Phenotype of Nonadherent Cells from Cocultures of Transduced CB CD34+ Cells on MS5 Stroma (in Percent)
| Week 0a
|
Week 1
|
Week 2
|
||||
|---|---|---|---|---|---|---|
| MiGR1 | STAT5A(1*6) | MiGR1 | STAT5A(1*6) | MiGR1 | STAT5A(1*6) | |
| CD34 | 92 | 93 | 10 | 11 | 1–4 | 1–4 |
| CD38 | 64 | 35 | 59 | <1 | 84 | 18 |
| CD33 | 99 | 99 | 84 | 8 | 78 | 17 |
| CD36 | 34 | 65 | 31 | 57 | 24 | 47 |
| CD71bright | 29 | 35 | 15 | 65 | 13 | 26 |
| CD45 | 99 | 99 | 88 | 14 | 95 | 7 |
| GPA | <1 | 7.5 | 10 | 64 | 5 | 41 |
| CD11b | 11 | 6 | 53 | 3 | 68 | 14 |
| CD14 | <1 | <1 | 45 | 4 | 27 | 2 |
| CD15 | <1 | <1 | 12 | 0 | 23 | 3 |
a
Week 0 represents analysis after 48 h of prestimulation followed by 48 h of transduction of CB CD34+ cells in QBSF with KL, FL, and TPO (100 ng/ml of each) before plating cells on MS5 stroma. Data represent the average percentages of two to five independent experiments.