Table II.
Generation of myeloid cells and DCs in vivo by CMP subpopulations
| CMP subpopulations | Donor-derived cells (×104)
|
||
|---|---|---|---|
| Injected (×104) | Mac-1+ Gr-1+ cells in BMa | Mac-1+ Gr-1+ cells per spleen | CD11c+ DCs per spleen |
| Flt3+ PU.1hi | 3.1–16.0 | 52.0–78.1 | 26.5–59.2 |
| Flt3− PU.1hi | 7.9–8.5 | 34.4–52.9 | 3.1–8.5 |
| Flt3− PU.1lo | 0.4–0.6 | 2.9–15.9 | 0.1–0.5 |
Purified BM progenitor populations from PU.1 gfp/gfp mice (C57BL/6 Ly5.2) were intravenously injected into lethally irradiated Ly5.1 recipient mice. For myeloid cell generation, the recipient mice were analyzed 10 d after injection. For DC generation, recipient mice were analyzed 14 d after injection. The values in this table are the ranges of donor-derived cell numbers obtained from two experiments. Each experiment included two to three recipients for each precursor population.
a
Donor-derived cells in the BM of two femur and two tibia collected from each mouse.