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. 1999 Jun 22;96(13):7575–7580. doi: 10.1073/pnas.96.13.7575

Figure 1.

Figure 1

Abnormal morphology of T1 transgenic rice plant carrying antisense RGA1 and their seeds (T2 seeds). (A) Morphology of the transgenic rice (4 mo old) and its seeds. Individual transgenic plants produced independently are designated as Anti α-1, Anti α-4, Anti α-5, and so on; Anti α, antisense RGA1 (cDNA for the α subunit of rice heterotrimeric G protein). Only the numbers of the individual transgenic plants are indicated below the plant pictures. Dwarf, dwarf plants; NC-like, apparently normal-cultivar-like plants; NC, normal cultivar (Nipponbare); IG, a transgenic plant expressing β-glucuronidase, a control for the transgenic plants with antisense RGA1. The white arrows indicate flowering (heads). T2 seeds from the transgenic plants are shown under the respective plants. (B) Northern blot analysis. See A for the numbers and NC. Total RNA (10 μg) from whole plants was loaded onto each lane. (Upper) The antisense RNA that is indicated by an arrow. (Lower) The sense RNA (endogenous mRNA) for the α subunit that is indicated by an arrow. (C) Southern blot analysis. See A for the numbers and NC (Upper). The arrow shows the α-subunit gene in the normal cultivar.