Skip to main content
. 1999 Jun 22;96(13):7575–7580. doi: 10.1073/pnas.96.13.7575

Figure 4.

Figure 4

Map of the α-subunit gene on chromosome 5 in normal cultivar and accumulation of the endogenous mRNA for the α subunit in alleles of Daikoku dwarf (d-1). (A) Map on chromosome 5 on the basis of the result from the Rice Genome Research Program. cM, centimorgan. 1, RFLP markers used in this study are indicated on the right side of chromosome 5. The position of the α-subunit gene was mapped between RFLP markers P71 and G1458. The distances between P71 and RGA1 and between RGA1 and G1458 are approximately 3.5 cM and 6.4 cM, respectively. 2, The approximate locus of Daikoku dwarf (d-1) cited from the data by Yoshimura et al. (27). RFLP markers are indicated on the right side of chromosome 5. The RFLP marker XNpb81 is identical with RFLP marker G81 used in map 1. The distances between XNpb81 and d-1 and between G81 and RGA1 are approximately the same (36 cM and 40 cM, respectively). (B) Analysis of Daikoku dwarf (d-1). 1, Nipponbare (a normal cultivar); 2, Daikoku dwarf (d-1), DK22, from the recurrent parent, Nipponbare; 3, Shiokari (a normal cultivar); 4, Daikoku dwarf (d-1), ID-1, from the recurrent parent, Shiokari. (Top) Morphology of flowering plants. The white arrows indicate flowering (heads). (Middle) Morphology of their seeds. (Bottom) Northern blot analysis. Poly(A)+ RNA (2 μg) from whole plants was loaded on each lane and hybridized with the DIG-labeled antisense RNA (pGEM-RGA1) as a probe. The sense RNA (endogenous mRNA) for the α subunit is indicated by an arrow.