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. 2004 Jun 21;199(12):1679–1688. doi: 10.1084/jem.20032121

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Copyright © 2004, The Rockefeller University Press

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Figure 1. — Frequency of circulating CD4⁺CD25⁺ T cells before and 1 wk after in vivo challenge with cow's milk. (A) PBMCs were purified from blood before and after the challenge, immunostained with mAbs, and then analyzed with FACS^® Caliber. The M1 gates were set in accordance with isotype- and concentration-matched mAb controls. One representative experiment is shown for the tolerant group (n = 10) and one for the allergic group (n = 7). (B) Increase or decrease of circulating CD4⁺CD25⁺ T cells 1 wk after in vivo exposure to cow's milk normalized in relation to the prechallenge levels (set to 100%, dotted line). Student's t test was used for statistical analysis.