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. 2004 Jun 21;199(12):1641–1650. doi: 10.1084/jem.20040520

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Requirement of TBK1 for IFN-inducible gene expression in virus-infected EFs. (A) Control (IKK-i ^+/− or TBK1^+/−), IKK-i ^−/−, or TBK1^−/− EFs were infected with recombinant VSV or SeV for the indicated periods. Total RNA was extracted and subjected to Northern blot analysis with probes for the indicated genes. Similar results were obtained from three independent experiments. (B) Nuclear translocation of IRF-3 and NF-κB p65 in response to VSV infection. EFs were infected with recombinant VSV for the indicated periods, and nuclear proteins were extracted, separated by SDS-PAGE, and blotted with anti-IRF-3 and NF-κB p65 Abs.