Figure 2.

CTLA-4^−/− DO.11 T cells cause severe insulitis in RIP-mOVA/Rag^−/− mice. Naive wild-type or CTLA-4^−/− DO.11 T cells (10 million per mouse) were transferred into RIP-mOVA/Rag^−/− recipients and immunized with OVA peptide and IFA where indicated. The mice were killed 13 d after transfer, and the pancreas were either formalin fixed and stained with hematoxylin and eosin (left) or frozen, acetone fixed, and stained with KJ1-26 (right) and observed under 40× magnification. Wild-type DO.11 T cells (A and B); wild-type DO.11 T cells and immunized with OVA peptide + IFA (C and D); CTLA4^−/− DO.11 T cells (E and F). Representative sections for each experimental group are shown.