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. 2004 Jun 21;199(12):1651–1658. doi: 10.1084/jem.20040528

Figure 3.

Figure 3.

TBK1-deficient BMMs induce normal NF-κB responses to LPS and polyI:C. TBK1+/+TNFR1+/− and TBK1−/−TNFR1−/− BMMs were stimulated with 10 ng/ml LPS or 1 μg/ml polyI:C for the indicated time points. (A) Nuclear fractions were probed for p65 and USF2 as a loading control. (B) EMSA was performed by incubating nuclear extracts were with an NF-κB-specific oligonucleotide. (C) Total RNA was extracted and analyzed by Q-PCR for expression of ICAM1 and IκBα.