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. 2004 Jun 21;199(12):1651–1658. doi: 10.1084/jem.20040528

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — TBK1-deficient BMMs have normal IFN responses to SeV. TBK1^+/+TNFR1^+/− and TBK1^−/−TNFR1^−/− BMMs were infected with SeV for the indicated time points. (A) Nuclear fractions were probed for IRF3 and USF2 as a loading control. (B) Total cell extracts were probed for phospho-STAT1 and total STAT1. (C) Total RNA was extracted and analyzed by Q-PCR for expression of IFNβ, IP-10, IFNα5, IRF7, IL-15, and Mx1.