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. 2004 Jun 21;199(12):1651–1658. doi: 10.1084/jem.20040528

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — TBK1-deficient MEF cells have impaired IFN responses to SeV. Wild-type and TBK1^−/− MEF cells were infected with SeV for the indicated time points. (A) Nuclear fractions were probed for IRF3 and USF2 as a loading control. Total cell extracts were probed for phospho-STAT1 and total STAT1. (B) Total RNA was extracted and analyzed by Q-PCR for expression of IFNβ, IP-10, IRF7, and IL-15. (C) EMSA was performed by incubating nuclear extracts were with an NF-κB-specific oligonucleotide. (D) Nuclear fractions were probed for p65 and USF2. (E) Total RNA was extracted and analyzed by Q-PCR for expression of ICAM1 and IκBα.