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. 2004 Jun 21;199(12):1651–1658. doi: 10.1084/jem.20040528

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Copyright © 2004, The Rockefeller University Press

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Figure 6. — IKK-i is differentially expressed in BMMs and MEFs, and can rescue a TBK1 deficiency during SeV infection. (A) Total RNA extracted from BMMs, wild-type MEFs, and TBK1^−/− MEFs were probed by Northern blot for expression of TBK1 and IKK-i. 28 and 18 sRNA bands are shown as loading controls. (B) Wild-type MEFs, TBK1^−/− MEFs, and TBK1^−/− MEFs reconstituted with wild-type TBK1 or IKK-i, or their kinase-inactive (KA) mutants were infected with SeV for the time points indicated. Nuclear fractions were probed for IRF3, p65, and USF2 as a loading control. Total cell extracts were probed for phospho-STAT1 and total STAT1. (C) Cells were infected with SeV as in B and total RNA was extracted and analyzed by Q-PCR for expression of IFNβ.