Table II.
Effect of Bcl2 overexpression and RAG1+/− mutation in donor cells on receptor editing and tolerance induction in κ-macroself radiation chimeras
| Percent positive mean ± SD |
||||||||
|---|---|---|---|---|---|---|---|---|
| Donor → Host (n)
|
Cells /106
mean ± SD |
B220
|
IgM
|
κ
|
λ
|
κ+B220int
|
λ+B220int
|
|
| Bone marrow | ||||||||
| Wild type | → wt (5) | 68 ± 6.6 | 78 ± 13 | 15 ± 2 | 21.0 ± 2.0 | 2.7 ± 0.5 | 12.8 ± 2.0 | 1.7 ± 0.1 |
| → Tg#2 (4) | 50 ± 13 | 74 ± 9 | 8 ± 3a
|
5.2 ± 1.8a | 4.9 ± 1.4a | 4.4 ± 1.3a | 2.7 ± 0.3a | |
| Bcl2 | → wt (4) | 56 ± 13 | 84 ± 5 | 35 ± 2 | 33.0 ± 7.0 | 8.6 ± 1.2 | 22.5 ± 3.0 | 9.3 ± 0.6 |
| → Tg#2 (3) | 61 ± 17 | 74 ± 8 | 22 ± 2a | 5.7 ± 0.4a | 15.0 ± 2.5a | 4.5 ± 0.4a | 15.0 ± 3.0a | |
| RAG1+/− | → wt (4) | 52 ± 7 | 67 ± 11 | 16 ± 2 | 21.0 ± 3.0 | 2.3 ± 0.7 | 9.7 ± 0.4 | 1.5 ± 0.4 |
| → Tg#2 (3) | 42 ± 2 | 60 ± 10 | 6 ± 2a | 8.3 ± 2.0a | 2.5 ± 0.7 | 7.4 ± 0.5a | 1.3 ± 0.2 | |
| Spleen | ||||||||
| Wild type | → wt (5) | 67 ± 23 | 66 ± 3 | 50 ± 2 | 5.0 ± 6.0 | 4.0 ± 1.0 | ||
| → Tg#2 (4) | 43 ± 7a | 36 ± 7a | 28 ± 5a | 0.6 ± 0.3a | 27.0 ± 7.0a | |||
| Bcl2 | → wt (4) | 146 ± 33 | 84 ± 3 | 63 ± 14 | 65.0 ± 8.0 | 10.0 ± 2.0 | ||
| → Tg#2 (3) | 83 ± 20a | 73 ± 15 | 49 ± 10 | 1.0 ± 0.6a | 41.0 ± 5.0a | |||
| RAG1+/− | → wt (4) | 64 ± 6 | 68 ± 4 | 50 ± 3 | 61.0 ± 4.0 | 4.7 ± 0.5 | ||
| → Tg#2 (3) | 39 ± 18a | 29 ± 6a | 26 ± 3a | 0.2 ± 0.1a | 19.0 ± 7.0a | |||
Bone marrow sample data shows total cell counts, but percentages of B cell subsets are based on a lymphocyte gate. B220int refers to cells with intermediate levels of B220 and the indicated Ig light chain.
a
Items marked represent significant (P < 0.05) differences between cells maturing in transgenic #2 compared to wild-type littermate hosts. Items in bold represent significant (P < 0.05) differences associated with the mutant donor type, compared to wild-type donor type in the comparable host.