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. 2005 Mar 7;201(5):755–767. doi: 10.1084/jem.20040429

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Sarcoidosis tissues extracted with sarkosyl contain poorly soluble protein antigens that bind to sarcoidosis IgG. (A) Protein fractions from sarcoidosis (S) or control (C) spleen. (B) Protein fractions from sarcoidosis lung. Protein fractions were analyzed by protein immunoblotting using purified IgG from sarcoidosis sera detected with anti–human Fcγ reagents. The initial homogenates (P22), intermediate supernatant fractions (S215, S1, and S2), or final cell pellets that were poorly soluble in sarkosyl (Pe) are shown. Pe were solubilized using 8 M urea plus β-mecaptoethanol and were precipitated before analysis by gel electrophoresis. Arrows point to antigenic bands seen in the Pe fractions of sarcoidosis but not control tissues. Arrowhead points to a 56-kD band detected with anti-Fcγ reagents alone in the Pe fraction from sarcoidosis lung (B).