Table I.

Summary of antigenic bands from protein immunoblots of extracts of sarcoidosis and control tissues

				Detecting sera
Sample		Pathology	Extraction	Sarcoidosis	Control	Antigenic bands (kD)	Anti-mKatG (IT57)
Sarcoidosis
S1	LNa	NCGb	TX100	−c	−	none	−
S2	LN	NCG	TX100	+	−	60	+
S3	LN	NCG	TX100	+	ND	62, 64	−
S4	LN	NCG	TX100	−	ND	none	ND
S5	LN	NCG	TX100	+	−	62, 64	+
S6	LN	NCG	TX100	+	ND	62, 64, 80	−
S7	LN	NCG	TX100	+	ND	∼160	ND
S8	LN	NCG	TX100	+	−	62	ND
S9	LN	no grand	TX100	−	−	none	−
S10	SPLe	NCG	TX100	+	−	60, 62	+
S11	SPL	NCG	TX100	+	+	60, 62f, 64f, 150, 160
			Sarkosyl	+	+	60, 62f, 64f, 150, 160	+
S12	LUg	NCG	TX100	+	ND	∼160
			Sarkosyl	+	−	60, 62, ∼160	+
Controls
C1	LN	no pathh	TX100	−	−	none	ND
C2	LN	no path	TX100	+	−	62	−
C3	LN	no path	TX100	−	−	none	−
C4	LN	no path	TX100	−	−	none	−
C5	LN	no path	TX100	−	−	none	−
C6	LN	FHi	TX100	−	−	none	−
C7	LN	FH	TX100	−	ND	none	ND
C8	LN	no path	TX100	−	ND	none	−
C9	LN	no path	TX100	−	ND	none	−
C10	LN	no path	TX100	−	−	none	−
C11	LN	no path	TX100	−	ND	none	ND
C12	SPL	no path	TX100	−	ND	none	−
C13	SPL	no path	TX100	−	−	none	−
C14	SPL	no path	Sarkosyl	−	ND	none	ND
C15	SPL	no path	Sarkosyl	−	−	none	ND
C16	SPL	no path	Sarkosyl	−	−	none	ND
C17	SPL	no path	Sarkosyl	−	ND	none	−
C18	SPL	no path	TX100	−	−	none	−
C19	SPL	no path	TX100	−	ND	none
			Sarkosyl	+	+	62f, 64f	−
C20	SPL	no path	Sarkosyl	−	−	none	ND
C21	SPL	no path	TX100	+	ND	62, 64	ND
C22	LU	no path	TX100	−	ND	none	−
			Sarkosyl	−	ND	none	−

^aLymph node.

^bNoncaseating granuloma.

^c−, no clearly detectable band; +, presence of bands not seen with secondary antibody alone.

^dNo granuloma.

^eSpleen.

^fBands also seen with control sera.

^gLung.

^hNo tumor, granuloma, or other identified pathology.

ⁱFollicular hyperplasia.