Table II.
Summary of peptide matches for mKatG using MALDI-TOF mass spectrometry of sarcoidosis spleen tissuea
| m/z submitted | MH+ matched | δ ppm | Start | End | Peptide sequence |
|---|---|---|---|---|---|
| 562.5000 | 562.2949 | 364.7568 | 485 | 489 | (R)GSDKR(G) |
| 833.2000 | 833.4634 | −315.9844 | 687 | 693 | (K)VKWTGSR(V) |
| 1163.7000 | 1163.5420 | 135.7729 | 105 | 114 | (R)MAWHAAGTYR(I) |
| 1437.1000 | 1437.7603 | −459.2364 | 558 | 571 | (K)AAGHNITVPFTPGR(T) |
| 1475.9000 | 1475.7382 | 109.6406 | 706 | 719 | (R)ALVEVYGADDAQPK(F) |
| 1708.3000 | 1707.9295 | 216.9512 | 555 | 571 | (K)AAKAAGHNITVPFTPGR(T) |
| 1713.9000 | 1713.8124 | 51.0985 | 397 | 410 | (R)WLEHPEELADEFAK(A) |
| 1741.6000 | 1741.8345 | −134.6375 | 105 | 119 | (R)MAWHAAGTYRIHDGR(G) |
| 1774.3000 | 1774.7938 | −278.2053 | 28 | 42 | (K)YPVEGGGNQDWWPNR(L) |
| 1869.8000 | 1869.9135 | −60.7182 | 396 | 410 | (R)RWLEHPEELADEFAK(A) |
| 1901.1000 | 1901.9510 | −447.4279 | 129 | 145 | (R)FAPLNSWPDNASLDKAR(R) |
| 1947.0000 | 1947.9664 | −496.0873 | 520 | 537 | (R)TLEEIQESFNSAAPGNIK(V) |
| 2018.9000 | 2018.0963 | 398.2698 | 596 | 613 | (R)NYLGKGNPLPAEYILLDK(A) |
| 2342.3000 | 2343.1517 | −363.5002 | 159 | 179 | (K)LSWADLIVFAGNCALESMGFK(T) |
| 2445.2000 | 2446.1574 | −391.3758 | 120 | 143 | (R)GGAGGGMQRFAPLNSWPDNASLDK(A) |
| 2470.5000 | 2471.2467 | −302.1548 | 158 | 179 | (K)KLSWADLIESMGFK(T) |
a
Peptides from the high salt precipitate are shown if both low and high salt precipitates resulted in peptide peaks of similar size (<1 dalton).