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. 2005 Mar 7;201(5):713–722. doi: 10.1084/jem.20042031

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Properties of RvE1. (a) Total organic synthesis of RvE1. Precursors 1–3 were prepared in isomerically pure form from starting materials with known stereochemistry and coupled sequentially to form acetylenic intermediate compound 4, which was selectively hydrogenated to form isomerically pure RvE1. (b) MS/MS spectrum of synthetic RvE1. (c) Inhibition of leukocyte trafficking in murine dorsal air pouch. 18R-HEPE and RvE1 (biogenic and synthetic) are at 100 ng/mouse, and dexamethasone is at 10 μg/mouse. Values represent mean ± SEM from five different mice. *, P < 0.05 (vs. vehicle control).