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. 2005 Mar 7;201(5):769–777. doi: 10.1084/jem.20041179

Figure 2.

Figure 2.

CD4+CD25+ Treg cells extensively proliferate in the lymph nodes of neonatal mice. Newborn C57BL/6 IL-2Rβ−/− mice received purified CD45.1+ CFSE-labeled CD4+CD25+ T cells (4–5 × 105) and 2–20 d after transfer, spleen (SP), and lymph node (LN) cells from individual recipient mice were subjected to FACS analysis. (A) Dot plots are shown for CD45.1 and CD4 expression (top) or CD25 and CFSE expression for CD45.1+CD4+ donor cells (bottom). The percent of donor CD4+ cells is indicated in the upper right corner of top dot plots. A total of 105 or 106 splenic cells were collected from 2- or 4-d-old mice, respectively. (B) Dot plots are shown for CD45.1 and CFSE expression. The percent of donor cells is indicated in the upper right corner of each dot plot. (C) Dot plots depicting CD4 and CD25 expression for CD45.1+ donor cells. The percent of cells expressing CD4 and CD25 of the gated populations is indicated in the upper right corner of the dot plots. Data in A and B are representative of two to five animals per day.