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. 2005 Aug 15;202(4):541–549. doi: 10.1084/jem.20041503

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — CD4⁺ T cells in the K5-TSLP mice express cutaneous homing receptors. Flow cytometry analysis of homing receptor expression by CD4⁺ T cells isolated from a K5-TSLP mouse and an NLC after 5 wk of dox treatment. Data are representative of more than five animals analyzed in this manner. (A) P-ligand (P-lig) and E-ligand (E-lig) expression was assessed by binding to P- and E-selectin–IgM fusion proteins in conjunction with intracellular cytokine staining to detect expression of IL-4. The percentages of cells in each quadrant are shown. Per., peripheral. (B) RT-PCR analysis of chemokine expression in the skin of a K5-TSLP mouse and NLC after 3 wk of dox treatment. From the K5-TSLP mouse, total RNA isolated from affected lesional skin (L) and unaffected skin (U) were analyzed separately. The levels of mRNA of CCL17, CCL22, and β-actin were determined by semiquantitative RT-PCR analysis with threefold serial dilution of the template cDNA. (C) CCR4 and CCR7 expression assessed by binding to CCL22- and CCL19-IgG3 fusion proteins in conjunction with staining for L-selectin (L-sel). The percentages of cells in each quadrant are shown.