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. 2005 Aug 15;202(4):529–539. doi: 10.1084/jem.20050183

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Purified MTP transfers a lipid to recombinant CD1d in vitro. (A) 2 μg BSA or recombinant CD1d was coated onto microtiter wells and incubated with either lipid vesicles alone or lipid vesicles plus 0.5 μg purified MTP. Lipid vesicles contained either PE:NBD or TG:NBD. Donor PE vesicles were composed of 450 nmol unlabeled PE and 14 nmol PE:NBD per milliliter. Results are representative of six independent experiments. *, P < 0.01. (B) 2 μg of recombinant CD1d was coated onto microtiter wells and incubated with PE:NBD vesicles and increasing concentrations of purified MTP. *, P < 0.005; **, P < 0.001 compared with 0 μg MTP. Values are ±SD.