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. 2005 Sep 5;202(5):637–650. doi: 10.1084/jem.20050821

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Copyright © 2005, The Rockefeller University Press

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Figure 6. — Adoptively transferred WT and IFN-IR⁰ P14 CD8 T cells were maintained at an equal ratio either in naive or in LCMV immune hosts. 0.8 × 10⁶ each of WT (Thy1.2, Ly5.1) and IFN-IR⁰ (Thy1.2, Ly5.2) P14 CD8 T cells were transferred into (A) naive B6 Thy1.1 mice or into (B) LCMV-immune Thy1.1 mice that had previously received equal numbers of WT plus IFN-IR⁰ CD8 T cells before LCMV infection. WT/IFN-IR⁰ donor CD8 T cell ratios were analyzed at days 1 and 8 after transfer. The donor cells were identified based on positive staining for Thy1.2 as well as CFSE (R1 gate). In B, freshly transferred Thy1.2 donor CD8 T cells were distinguished (R1 gate) from previously primed Thy1.2 memory CD8 cells (R2 gate) based on the CFSE label. The numbers in parenthesis indicate the average recovery of donor cells per spleen (n = 3) in each group. Numbers represent the percentage of cells in the indicated quadrant.