Figure 8.
IFN-I action on a variety of CD8 T cell clonal specificities is required for their expansion in response to viral infection, cross priming, and vaccination in the presence of adjuvants that mimic viral infection–induced cytokine profiles. (A) 1.3 ×106 each of WT (Thy1.2, Ly5.1) and IFN-IR0 (Thy 1.2, Ly5.2) OT-1 CD8 T cells were transferred into C57BL/6 Thy1.1 mice. The mice were analyzed the next day (No infection), 5 d after LCMV infection alone (LCMV only), or 5 d after LCMV + ovalbumin immunization (LCMV + Ova). Data are gated on CD8 T cells. Numbers inside the box indicate the average ratio of IFN-IR0 (Thy1.2+ Ly5.1low) to WT (Thy1.2+ Ly5.1+) OT-1 CD8 T cells (n = 5 per group combined from two experiments). Similar trends were seen in a different experiment in which 4 × 106 each of the donor cells were transferred. (B) Average recovery of WT and IFN-IR0 OT-1 donor CD8 T cells per spleen in the experiments indicated in A. Values shown are the mean ± SD. (C) 3.4 × 106 each of enriched polyclonal CD8 T cells derived from age-matched C57BL/6 WT (Thy1.2, Ly5.1) and IFN-IR0 (Thy1.2, Ly5.2) mice were transferred into C57BL/6 Thy1.1 recipients and then infected with LCMV the next day. Mice were analyzed on day 5 after infection (day 6 after cell transfer in the case of uninfected recipients). Similar trends were observed in a different experiment in which mice were analyzed on day 6 after infection. (D) 500,000 each of WT (Thy1.2, Ly5.1) and IFN-IR0 (Thy1.2, Ly5.2) P14 CD8 T cells were transferred into B6 Thy1.1 hosts and left untreated, immunized with 25 μg GP33-41 peptide mixed with low (105 units) or high (106 units) dose universal IFN-I (IFN-α), immunized with peptide + 1 μg recombinant IL-12, or infected with LCMV. Dot plots show the average ratio of the IFN-IR0 versus WT donor P14 cells among the gated donor CD8 T cell population. (E) Recovery of IFN-IR0 and WT donor P14 CD8 T cells per spleen in the experiment described in D. Each point represents an individual mouse.