Figure 1.

Expression analysis of the K⁺ channel AKT3. (A) Northern blot analysis of 10 μg of total RNA isolated from mesophyll protoplasts and major veins of A. thaliana leaves. Hybridizations with ³²P-labeled probes are indicated on the right. The 18S probe demonstrated that equal amounts of total RNA were loaded in each lane. (B) Whole-mount hybridization of 7-week-old Arabidopsis plantlets grown on agar. (C) In situ hybridization of handmade cross sections from flower stalks of 10-week-old Arabidopsis plants grown on soil. Strand-specific antisense (Left) and sense (Right) cDNAs labeled with digoxigenin were used as probes.