Table II.
MMP-2 interacting molecules expression by target cells
| Expression of
|
||||||
|---|---|---|---|---|---|---|
| αvβ3
|
||||||
| Target cells | M134.12 recognition (TNF production) |
CD91 (Ab staining) |
MT1-MMP (RT-PCR) |
β3 chain (RT-PCR) |
Ab staining (RFI) |
|
| % | ||||||
| Melanoma cells | M17 | + | ND | +/− | 84 | 6.6 |
| M113 | + | ND | + | 182 | 4.6 | |
| M119 | + | ND | ND | 34 | 3.7 | |
| M134 | + | +/− | + | 100 (ref.) | 3.5 | |
| M147 | + | +/− | + | 57 | 2.5 | |
| M153 | + | +/− | +/− | 113 | 4.7 | |
| M204 | + | − | + | 67 | 7.6 | |
| FM25 | + | ND | + | 126 | 1.8 | |
| FM29 | + | + | + | 37 | 6.3 | |
| IPC 277/5 | + | ND | +/− | 143 | 3.6 | |
| M71 | − | ND | ND | 54 | 3.3 | |
| M74 | − | ND | ND | 14 | 3.5 | |
| M88 | − | +/− | + | 6 | 1.1 | |
| M101 | − | ND | ND | 7 | 1.3 | |
| M117 | − | +/− | + | 249 | 1.3 | |
| M102 | − | − | + | 96 | 3.2 | |
| Nonmelanoma tumor cells |
1355 | − | +/− | − | 4 | 1 |
| Bla-2 | − | ND | ND | 4 | 2 | |
| Heu-n | − | ND | ND | 0 | 1 | |
| LAN-1 | − | ND | ND | 0 | 1 | |
| HCT116 | − | ND | ND | 0 | 1 | |
| Sw480 | − | − | − | 0 | 1 | |
| Sw707 | − | ND | ND | 0 | 1 | |
| A498 | − | + | + | 58 | 5.5 | |
| R28 | − | ND | + | ND | ND | |
| OVCAR | − | ND | − | 5 | 1 | |
| TT | − | ND | − | 0 | 1 | |
| Normal cells | 98M09 (P6) | − | ND | ND | ND | 1.3 |
| 01M08 (P4) | − | ND | ND | ND | 1.2 | |
| MHN 3 (P1) | − | ND | ND | ND | 1.1 | |
| MHN 5 (P2) | − | ND | ND | ND | 1 | |
| K1 | − | ND | ND | ND | ND | |
| MG | − | ND | ND | ND | ND | |
| HFFF2 | − | ND | ND | ND | 1 | |
| F257 | − | ND | ND | 5 | 1.2 | |
| HAEC#8186 | − | ND | ND | ND | ND | |
| 10C10 | − | ND | ND | 0 | 1 | |
| M77.84 | − | ND | ND | 0 | 1 | |
| M17.221 | − | ND | ND | 0 | 1 | |
| M101.70 | − | ND | ND | 0 | 1 | |
Tumor and normal human HLA-A*0201+/MMP-2+cell lines and the M102 melanoma cell line (HLA-A*0201+/MMP-2−) were tested for MMP-2 interacting molecule expression by RT-PCR (MT1-MMP and β3) or by antibody staining (anti-αvβ3 [23C6] and anti-CD91 [A2MR-α2]). For β3, PCR band intensities were measured individually from the original TIFF image with NIH image-J software after normalization with β-actin. Results are expressed as a percentage of the level of expression in M134 melanoma cell line. To evaluate the level of αvβ3 expression, we determined RFI values as described in Material and methods. See Table I for the cell nature and origin.