Figure 3.

In vitro Th1 and Th2 cell generation by dendritic cells. 1 × 10⁴ purified DO11.10 CD4 T cells were cultured with 3 ×10⁴ BMDCs from WT or B7-DC KO mice with OVA_323-339 peptide for 5 d in the presence of recombinant IL-12 and anti-IL-4 mAb for Th1 generation or recombinant IL-4 and anti-IFN-γ mAb for Th2 generation. 6 h before end of culture, additional OVA_323-339 peptide with or without anti-CD3 mAb was added for restimulation. After surface CD4 was stained, cells were fixed and permeabilized, and intracellular IFN-γ and IL-4 were stained. The data shown are gated on CD4⁺ cells. Data are representative of four independent experiments.