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. 2005 May 16;201(10):1531–1541. doi: 10.1084/jem.20050072

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — In vivo CD4 T cell stimulation after DC immunization. 5 ×10⁶ purified CFSE-labeled DO11.10 CD4 T cells were transferred into WT or B7-DC KO mice on d −1. Mice were then immunized with 1 ×10⁶ OVA_323-339 peptide–pulsed BMDCs on d 0. Peptide-pulsed BMDCs from WT mice were injected into WT mice, and peptide-pulsed BMDCs from B7-DC KO mice were injected into B7-DC KO mice, respectively. (a and b) Proportion and (c and d) cell division (CFSE dilution) of CD4⁺KJ1-26⁺ T cells in spleen, PLN, liver, and HLN were analyzed by FACS on d 3 (a and c) and d 5 (b and d). The CFSE-labeled CD4⁺KJ1-26⁺ T cells in liver, immunized with BMDCs without OVA_323-339 peptide, are also shown in Fig. S2 as negative controls. (e) The absolute number of DO11.10 CD4 T cells in spleen and liver was calculated on d 3 and 5 by the formula: total cell number × percent CD4⁺ cells × percent CD4⁺ cells staining with KJ1-26. *, Significant difference (P < 0.001) observed in liver of six pairs of B7-DC KO mice and WT mice on d 3 and 5. Data are representative of three independent experiments.

Figure 4. — In vivo CD4 T cell stimulation after DC immunization. 5 ×10⁶ purified CFSE-labeled DO11.10 CD4 T cells were transferred into WT or B7-DC KO mice on d −1. Mice were then immunized with 1 ×10⁶ OVA_323-339 peptide–pulsed BMDCs on d 0. Peptide-pulsed BMDCs from WT mice were injected into WT mice, and peptide-pulsed BMDCs from B7-DC KO mice were injected into B7-DC KO mice, respectively. (a and b) Proportion and (c and d) cell division (CFSE dilution) of CD4⁺KJ1-26⁺ T cells in spleen, PLN, liver, and HLN were analyzed by FACS on d 3 (a and c) and d 5 (b and d). The CFSE-labeled CD4⁺KJ1-26⁺ T cells in liver, immunized with BMDCs without OVA_323-339 peptide, are also shown in Fig. S2 as negative controls. (e) The absolute number of DO11.10 CD4 T cells in spleen and liver was calculated on d 3 and 5 by the formula: total cell number × percent CD4⁺ cells × percent CD4⁺ cells staining with KJ1-26. *, Significant difference (P < 0.001) observed in liver of six pairs of B7-DC KO mice and WT mice on d 3 and 5. Data are representative of three independent experiments.