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. 2005 May 16;201(10):1647–1657. doi: 10.1084/jem.20041044

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Gut-specific expression of CD103 by hsCD8eff is dependent on TGF-β activity. (A) Lethally irradiated BALB/c (H-2^d) hosts were adoptively transferred with 10⁶ SC from either 2C TCR-Tg mice expressing 2C-DNR in combination with BMC and SC from B6 (H-2^b) mice. Recipients were killed at day 7 after BMT and lymphocytes infiltrating the intestinal epithelium were subjected to a three-color FACS analysis. Data shown are histograms of CD44 (left) or CD103 (right) expression by gated host-specific CD8⁺1B2⁺ (y axis, events). (B) Lethally irradiated BALB/c (H-2^d) hosts were adoptively transferred with an equal mixture (0.5 × 10⁶) of SC from wild-type 2C (Thy1.1⁺) and 2C-DNR (Thy1.1⁻) mice in combination with BMC and SC from B6 (H-2^b) mice. Lymphocytes infiltrating the intestinal epithelium were isolated at the indicated time points and subjected to a three-color FACS analysis. (left) dot plot shows Thy1.1 and 1B2 expression by gated CD8⁺ lymphocytes. (right) histograms show the percentage of CD103 expression by gated host-specific CD8⁺1B2⁺ cells of either a wild-type 2C (top, Thy1.1⁺1B2⁺ cells) or 2C-DNR (bottom, Thy1.1⁻1B2⁺ cells) origin (y axis, events). Isotype control staining is indicated by shaded histograms. Results are representative of three independent experiments.