Table II.
Reactivity of Vδ2neg γδ T cells against tumor epithelial cells is independent of MICA expression
| TNFα production (pg/ml)a
|
||||||||
|---|---|---|---|---|---|---|---|---|
| MICAneg cell lines
|
MICApos cell lines
|
|||||||
| Clones | V segment usage | SK-CO1 | HUH-7 | HUTU-80 | SK-MES | HeLa | HT-29 | Caco-2 |
| 2-6 | Vγ2,3 Vδ1 | <5 | 70 | 414 | 7 | 40 | 98 | 103 |
| 4-13 | Vγ4 Vδ5 | <5 | <5 | <5 | <5 | 15 | 220 | 333 |
| 4-29 | Vγ4 Vδ5 | 70 | 91 | <5 | <5 | 281 | 546 | 615 |
| 5-4 | Vγ9 Vδ1 | 105 | 34 | 30 | <5 | 388 | 428 | 543 |
| 5-15 | Vγ8 Vδ1 | 8 | 109 | 324 | 223 | 344 | 54 | 36 |
a
3 × 104 cells/well of five different Vδ2neg γδ T cell clones were cultured for 4 h on monolayers of seven tumor epithelial cell lines expressing MICA or not. TNFα concentration was measured in culture supernatant by ELISA. Results are the mean of culture triplicates and SD was always <15% of the mean value (unpublished data).