Table I.
Bacterial strains and used in this study
| Plasmid/strain | Properties | Origin/reference |
|---|---|---|
| L. lactis cremoris 1363 | Prophage-cured L. lactis cremoris strain | (52) |
| L. lactis ClfA-positive | L. lactis strain expressing S. aureus ClfA | (47) |
| L. lactis FnBPA-positive | L. lactis strain expressing S. aureus FnBPA | (18) |
| L. lactis LPXTG |
L. lactis strain expressing the cell wall-anchoring domain of S. aureus FnBPA (amino acid 881–1018) |
This study |
| L. lactis CD |
L. lactis strain expressing domains C and D of S. aureus
FnBPA (amino acid 606–1018) |
This study |
|
L. lactis A-ClfA plus CD-FnBPA |
L. lactis strain expressing domain A of S. aureus ClfA (amino acid 1-502) fused to domains C and D of S. aureus FnBPA (amino acid 606–1018) |
This study (used for cis-supplementation) |
|
L. lactis ClfA plus CD-FnBPA |
L. lactis strain expressing whole S. aureus ClfA (as in L. lactis
ClfA-positive, above) plus domains C-D of S. aureus FnBPA (amino acid 606–1018) |
This study (used for trans-supplementation) |
| S. aureus Newman | ClfA-producing strain | (19) |
| S. aureus Cowan I | FnBPA and FnBPB-producing strain | ATCC 12598 |
| S. aureus 8325-4 | Prophage-cured ATCC strain low production of FnBPA and FnBPB |
(53) |
| S. aureus DU5883 | FnBPA and FnBPB-negative mutant | (54) |
ATCC, American Type Culture Collection.