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. 2005 Sep 19;202(6):733–738. doi: 10.1084/jem.20051378

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — m- and z-AID are catalytically active in E. coli and S. cerevisiae. (A) Mutator assay in E. coli. Expression of m-, z-, and f-AID was induced for 3 h in exponentially growing cultures that were then assayed for kanamycin resistance. Points represent the mutation frequency of individual starting colonies. The mean values and standard deviations from three experiments are given. (B) Mutator assay in ung ⁺ and ung ⁻ S. cerevisiae. Expression of m-, f-, and z-AID was induced by galactose for 48 h at 30°C, and cultures were assayed for ura3 auxotrophy in 5-FOA–containing medium. Points represent the mutation frequency in cultures from individual starting colonies. The mean values and standard deviations are given. ung ⁺ background data were from four experiments. One experiment in the ung⁻ background is shown.