Figure 6.

Tumor metastasis in L-selectin^−/− and FucT-IV^−/−/VII^−/− mice. (A–D) B16-F10 melanoma cells were inoculated s.c. into the right hind footpads and the development of tumor foci was examined 10 d later. Tumor formation was examined in 22 C57BL/6 (A), 11 Rag-1 ^−/− (B), 26 L-selectin^−/− (C), and 14 FucT-IV^−/−/VII^−/− (D) mice. Representative results for six mice from each group are shown. (E) The number of NK cells in the tumor-bearing popliteal lymph nodes. Bars indicate the median values. (F) The relative tumor burden in the popliteal lymph nodes bearing B16 tumors. Bars indicate the median values. (G) In vitro cytotoxicity of L-selectin^−/− (•) and wild-type (Δ) NK cells against B16-F10 tumors. One out of four independent experiments is shown. p-values are relative to C57BL/6 mice.