Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Dec 19;202(12):1669–1677. doi: 10.1084/jem.20051190

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — Recombination at endogenous IgH loci. (A) Bone marrow CLPs as in Fig. 5 D were examined for D-J_H rearrangement status at the endogenous IgH locus by PCR. Germline (GL) DNA and rearrangements of D to J₁, J₂, J₃, or J₄ is indicated by arrowheads. Data from two independent sorts is shown (E47 heterozygote no. 1 and E47 knockout no. 1, 100 CLPs each; E47 heterozygote no. 2 and E47 knockout no. 2, 150 CLPs each). Rearrangement status of whole bone marrow from wild-type B6 mice and from rag2 knockout mice is provided for comparison. Inset depicts the frequency of CLPs that have detectable D-J_H rearrangements as determined by quantitative single cell PCR. (B) rag1 expression was quantified by real-time RT-PCR using 350 cell equivalents of cDNA from CLPs. Expression of rag1 and the reference gene β-actin are shown for equal numbers of CLPs from E47-heterozygous and E47 knockout animals. ΔRN, the fluorescence intensity. Identical results were obtained using cells from at least two independent sorts.