Figure 2.

Role of TLR9 and TLR2 in proinflammatory cytokine production by M. tuberculosis–stimulated macrophages. BMM from WT, MyD88^−/−, TLR2^−/−, TLR9^−/−, and TLR2/9^−/− were stimulated with M. tuberculosis (MOI = 1:1), 15 μg/ml CpG, 5 μg/ml PGN, or 100 ng/ml LPS for 24 h. (A) IL-12p40, (B) TNF, and (C) IL-6 production was measured in the culture supernatants by ELISA. Results are means ± SE of triplicate measurements. Experiments shown are representative of two performed. *, P < 0.05 between WT versus KO values.