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. 2005 Jul 18;202(2):249–259. doi: 10.1084/jem.20041685

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Fas-mediated apoptosis in WR/Fas-SM(−) and WR/Fas-SMS1 cells. (A and B) Dose dependency of Fas-mediated apoptosis. Cells were incubated for 3 h with the indicated concentration of CH11. After incubation, cells were harvested and analyzed by flow cytometry for DNA fragmentation using nuclear staining with PI. The numbers in each box represent the percentages of apoptotic cells. (C) Time kinetics of Fas-mediated apoptosis. Cells were incubated with 50 ng/ml of CH11 for the indicated time, and apoptosis was analyzed by flow cytometry using PI. Error bars represent SEM. (D) Time–kinetics of loss of ΔΨm in apoptotic cells. Cells were incubated with 50 ng/ml of CH11 for the indicated time. ΔΨm was determined by intracellular staining with DiOC₆(3) and flow cytometry. (E) Inhibition of Fas-mediated apoptosis by caspase inhibitors. Cells were stimulated with 50 ng/ml of CH11 for 6 h in the presence of the indicated amounts of Ac-DEVD-CHO or Ac-IETD-CHO. After incubation, apoptosis was analyzed by flow cytometry using PI.