Table II.
Chemotaxis of blood-borne NP-specific B cells
| Migration as a percentage of inputa
|
||||
|---|---|---|---|---|
| Chemokineb | B220+IgMIgD+ | B220+IgMIgD− | B220+NP+IgG1+ | Non–B cells |
| % | % | % | % | |
| None | 0.09 ± 0.01 | 0.70 ± 0.14 | 0 | 0.95 ± 0.07 |
| CXCL13 | 8.8 ± 1.6 | 2.9 ± 0.7 | 15.0 ± 4.1 | 1.8 ± 0.3 |
| CXCL12 | 7.8 ± 6.1 | 4.8 ± 4.0 | 0.6 ± 0.8 | 5.3 ± 3.0 |
a
Cell populations were enumerated by flow cytometry before and after migration. The number migrating through the membrane is expressed as a fraction of input cells of that phenotype. Data are from two experiments each using four wells (CXCL13) and two wells (CXCL12).
b
CXCL13 present at 800 ng/mL; CXCL12 present at 400 ng/mL.