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. 2005 Feb 21;201(4):647–657. doi: 10.1084/jem.20041611

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Lentiviral vectors and phosphorylation of DAP12 after stimulation of TREM2. (A) Schematic drawing of lentiviral vectors for expression of fTREM2, GFP (GFP1), DAP12 tagged with GFP (DAP12-GFP), or mutant mtDAP12 tagged with GFP (mtDAP12-GFP). (B) Phosphorylated DAP12-GFP was detected after blotting with the antibody directed against tyrosine phosphate in fTREM2 plus DAP12-GFP–cotransfected cells and after cross-linking stimulation. DAP12-GFP or mtDAP12-GFP were detected after reblotting with the antibody directed against GFP in the samples, respectively.