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. 2005 Feb 21;201(4):637–645. doi: 10.1084/jem.20042066

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Pol η interacts with MSH2 in vitro and in vivo. (A) Coomassie-stained gel. An SDS-PAGE gel shows the purity of the GST-tagged COOH-terminal pols before they were coupled to glutathione-Sepharose beads. (B) GST pull-down assay. ³⁵S-labeled MSH2, MSH3, and MSH6 proteins were incubated with the pol-coupled beads and resolved on an SDS-PAGE gel. Input lanes represent 100% of the MSH protein added to the assay tube. (C) Immunoprecipitation. FLAG-tagged pol η was overexpressed in HeLa cells and immunoprecipitated (IP) with or without antibody to FLAG protein. Precipitates were solubilized and separated by electrophoresis. The gel was immunoblotted (IB) for Western analyses using an antibody to FLAG to detect pol η and an antibody to MSH2. Input lanes represent 30% of the cell extracts used in the assay.