Figure 2.

Bet.-APE induce DCs allostimulatory activity. (A) Immature DCs were left untreated (medium) or stimulated with LPS (100 ng/ml), Bet.-APE (3 mg/ml) alone, or together with LPS (100 ng/ml). After 24 h, DCs were analyzed for their capacity to induce T cell proliferation in naive allogeneic CD4⁺ CD45RA⁺ T lymphocytes (10⁵/well). (B) DCs (2.5 × 10⁴) stimulated with graded concentrations of Bet.-APE alone or together with LPS (100 ng/ml) were coincubated with naive T cells. T cell proliferation was measured by BrDU incorporation after 3 d of coculture. Results are given as mean OD ± SD of triplicate cultures and are representative of five independent experiments from different nonatopic donors.