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. 2005 Feb 21;201(4):509–515. doi: 10.1084/jem.20041310

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Expression of functional ChemR23 in blood DC subsets. Blood DC subsets were isolated as described in Materials and methods and stained for ChemR23 expression. Maturation of DCs was achieved by incubation with LPS (BDCA-1⁺), influenza virus (BDCA-4⁺), or LPS and influenza virus (BDCA-3⁺) for 24 h. (a) DC subsets were analyzed by FACS analysis. (b) DC subsets were tested for their ability to migrate across an endothelial cell monolayer in response to chemerin or to an optimal concentration (12 nM) of CXCL12 (immature DCs) or CCL19 (mature DCs). The values represent the mean ± SD of triplicate data points and are representative of at least five different experiments performed with independent donors. Note the different scale used in the left versus right panels. (iDC) Immature DCs. (mDC) Mature DCs. *, P < 0.05 by paired Student's t test.