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. 2005 Aug 1;202(3):345–351. doi: 10.1084/jem.20050645

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Copyright © 2005, The Rockefeller University Press

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Figure 2. — Lack of multinucleation in DC-STAMP^−/−osteoclasts. (A) TRAP expression was induced, but multinucleation was completely abrogated in DC-STAMP^−/− osteoclasts. TRAP staining (a) and the number of multinuclear TRAP-positive cells (b) are shown. Values represent SD. Bar, 50 μm. (B) TRAP solution assay of macrophages (M-CSF alone) and osteoclasts (M-CSF + RANKL). (C) Expression of c-fos, NFATc1, or cathepsin K in macrophages (M-CSF alone) or osteoclasts (M-CSF + RANKL) derived from DC-STAMP^+/+, DC-STAMP^+/−, or DC-STAMP^−/− mice was analyzed by RT-PCR. NC, no template control. (D) Ruffled border formation was detected in osteoclasts of DC-STAMP^−/− tibial sections under electron microscopy. RB, ruffled border. (E) Total number of nuclei in cultured osteoclasts. Values represent SD. (F) The percent frequency of a population of osteoclast precursor cells (boxes, c-Fms⁺c-Kit⁺Mac-1^low) is shown as the mean ± SD. (G) Resorbing lacunae were visualized by toluidine blue O staining (a), and relative resorbing areas were scored (b). Values represent SD.