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. 2005 Mar 21;201(6):937–947. doi: 10.1084/jem.20041901

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Differences in cell viability and apoptotic response after RV-16 infection in asthmatic and normal BECs. (A) Viable (AxV⁻/7AAD⁻) cell number was determined 8 h after infection and expressed as percent viability compared with cells treated with medium alone. Infection with RV-16 led to a significant reduction in median (IQR) cell viability in both asthmatic and control cells compared with both medium alone (P = 0.03 and 0.02, respectively) and UV-inactivated RV-16 (P = 0.02 and 0.001, respectively). In asthmatic cells there was significantly better viability, median 80% (74, 86), compared with healthy controls 63% (51, 69; P = 0.02). (B) Apoptotic (AxV⁺/7AAD⁻) cells were also analyzed 8 h after RV-16 infection. Data are expressed as median (IQR) fold change in apoptosis from baseline. There was a significant and virus-specific increase in cell apoptosis in response to infection in cells from both groups, however, this response was significantly impaired in asthmatic cells with a fold increase of only 1.4 (1.3, 1.7), compared with 2.2 (2.1, 2.3) in healthy controls (P = 0.02). (C) The time course for activation of caspase 3/7 by RV-16 was determined in cells from 10 ICSs requiring asthmatics and 10 healthy controls, all conditions were done in quadruplicate, values represent the fold induction from baseline. There was significant mean (SD) induction of active caspase 3/7 in response to infection in normal cells at 4, 8, and 12 h, induction reached a plateau at 8 h. In asthmatic cells the induction was later, being significantly increased above baseline only at 8 and 12 h and was of significantly reduced magnitude at each time point compared with normal cells. At 8 h there was a significantly impaired induction of active caspase 3/7 in asthmatic cells (mean [SD] = 1.47 [0.13]) compared with healthy controls (mean [SD] =2.16 [0.34]; P = 0.004). *, Significantly different from cells treated with UV-inactivated RV-16 and medium only (P < 0.01). **, Asthmatic cells significantly different from healthy controls (P < 0.05). +, Significantly different from baseline (P < 0.05). Shaded bars, asthma; open bars, healthy controls.